Copure® SLE

Supported Liquid Extraction (SLE) is a very efficient and economical sample preparation methodology. Extracting analytes from aqueous samples is achieved with a simple two-step protocol, i.e., loading and elution.

In SLE, porous diatomaceous earth with high surface area and low chemical activity is packed as stationary support for liquid-liquid partition. While loading a sample, the aqueous sample solution passes through diatomaceous earth beads, allowed to adsorb via capillary action, forming a thin membrane. Then, a small volume of organic solvent percolates and produces an aqueous-organic interface. Because the mass transfer occurs in extreme short paths, analytes can partition very efficiently between the two phases.

Features:

• High recoveries, more reproducible
• Simplified protocols, less time-cosuming
• No need to vigorous shaking, no emulsion formation
• Using less amount of organic solvents, reducing cost
• Easy to automation and parallel manipulation

Applications:

SLE is widely used in food safety, environmental protection, clinical diagnosis, forensic science and material inspection. For example:

• Analysis of drugs and drug metabolites in biological fluids, such as antidepressant sertraline and anti-inflammatory drug ibuprofen
• Determination of parabens in cosmetics, pharmaceuticals and foodstuffs.

Example: extracting ibuprofen in human serum using Biocomma SLE

Chromatographic conditions:

SLE cartridge: Biocomma SLE, 1 mL

System: Waters Alliance 2690

Column: Welch Ultimate XB-C18 (4.6x250 mm)

Mobile phase: 0.1% formic acid in water : acetonitrile = 20:80 (v/v)

Flowrate: 1 mL/min

Injection volume: 20 uL

Detection: UV at 220 nm

Results show that recoveries greater than 79.7% are achieved in extracting ibuprofen from human serum by using Bicomma SLE (1 mL).

Order information:

Product No.	Format	Qty.
SLE1CC	Copure ® SLE cartridges, 1 mL	100/PK
SLE3CC	Copure ® SLE cartridges, 3 mL	50/PK
SLE6CC	Copure ® SLE cartridges, 6 mL	30/PK
SLE12CC	Copure ® SLE cartridges, 12 mL	20/PK